AATCC 147 - Parallel Streak MethodvAntibacterial Activity Assessment of Textile Materials
AATCC 147 Test Summary:
• This is a 24 hour qualitative agar plate-based assay for the evaluation of antibacterial activity of diffusable antimicrobial agents on treated textile materials.
• Challenge microorganisms are used from fresh cultures.
• Standard tests follow the 5-streak protocol using all test microorganisms listed below.
• Standard contact time for this test is 24 hours. Custom contact times are welcome upon request.
• All tests run following GLP.
• Each test sample will be tested in triplicate to ensure precision.
AATCC 147 Test Sample:
• Samples can be submitted as 25 x 50 mm rectangles or as sheets for in-lab preparation.
• Untreated samples should be submitted for use as control samples. Like material matching can be provided if necessary.
AATCC 147 Test Organism:
Test Standards Recommend:
Gram-positive bacteria: Staphylococcus aureus, ATCC No. 6538.
Gram-negative bacteria: Klebsiella pneumoniae, ATCC No. 4352.
Alternate available organisms:
Escherichia coli ATCC-25922
Pseudomonas aeruginosa ATCC-9027
Canadida albicans ATCC-10231
*Other organisms can also be obtained per request.
Turn around Time: 5 to 7 days for commonly used organisms like mentioned in AATCC 147 test standard.
Description from AATCC:
Antibacterial finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but cumbersome and time consuming for routine quality control and screening tests. Therefore, when the intent is to demonstrate bacteriostatic activity by the diffusion of the antibacterial agent through agar, Method 147 fulfills this need. In the Parallel Streak Method, the agar surface is inoculated making it easier to distinguish between the test organism and contaminant organisms which may be present on the unsterilized specimen. The Parallel Streak Method has proven effective over a number of years of use in providing evidence of antibacterial activity against both Gram positive and Gram negative bacteria.
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AATCC-30-2004, Test III - Antifungal Activity, Assessment on Textile Materials: Mildew and Rot Resistance of Textile Materials